Published 2006
| Version v1
Dataset
Open
Caulobacter crescentus
- Researcher:
- Prabha Dias, D.
Description
wild type CB15N. 2 of 8, no warping. mid-divisional cell constriction site + stalk end; In Curv filament + FtsZ filaments; very dense 'medusa-head' feature; amorphous density near stalk
Files
SAVE_InCurv_xz_02.jpg
Files
(11.3 MB)
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Technical info
Tilt Series Date: 2006-06-22
Data Taken By: Prabha Dias Species / Specimen: Caulobacter crescentus Strain: NA1000 Tilt Series Settings: Single Axis, tilt range: (-61.0°, 61.0°), step: 0.7°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -12.0 μm, magnification: 0x. Acquisition Software: UCSF Tomo Upload Method: rundir Processing Software Used: imod, bsoft Collaborators and Roles: specimen prep, data collection & processing: D. Prabha Dias Purification / Growth Conditions / Treatment: standard PYE media (Shapiro, Brun, etc. recipe); cultured in 50ml flask; 5 days growth; see DPD notebook Jun8-12, 2006 Sample Preparation: box 694, grid 1; MeOH-washed Quantifoil R2/1; 10nm gold on grids & in suspension; offset -2, blot 1.5 sec, drain 1 sec; see DPD notebook Aug 14, 2006 SAVE_blackmass_xy_01.jpg: file associated to 3D Image #304 SAVE_blackmass_yz_01.jpg: file associated to 3D Image #304 SAVE_ftsZ_yz_01.jpg: file associated to 3D Image #304 SAVE_InCurv_xy_01.jpg: file associated to 3D Image #304 SAVE_InCurv_xy_02.jpg: file associated to 3D Image #304 SAVE_InCurv_xz_01.jpg: file associated to 3D Image #304 SAVE_InCurv_xz_02.jpg: file associated to 3D Image #304 pdd2006-06-22-2_slicer10039.jpg: Caulobacter crescentus . peptidoglycan . s-layer pdd2006-06-22-2_slicer10040.jpg: Caulobacter crescentus . extracellular vesicleExternal Files
Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pdd2006-06-22-2/rawdata/
06Jun22t02.mrc, Tilt Series (Pixel Size 1.2 nm), 1.5 GB Download 06Jun22t02bn_full.rec, Reconstruction (Pixel Size 2.4 nm), 671.1 MB Download trim.rec, Reconstruction (Pixel Size 2.4 nm), 10.5 MB Download 06Jun22t02_13kx.dm3, * associated to rawdata, 8.6 MB Download SAVE_blackmass_xy_01.tif, file associated to 3D Image #304, 6.0 MB Download SAVE_blackmass_yz_01.tif, file associated to 3D Image #304, 6.0 MB Download SAVE_ftsZ_yz_01.tif, file associated to 3D Image #304, 6.1 MB Download SAVE_InCurv_xy_01.tif, file associated to 3D Image #304, 6.1 MB Download SAVE_InCurv_xy_02.tif, file associated to 3D Image #304, 6.0 MB Download SAVE_InCurv_xz_01.tif, file associated to 3D Image #304, 6.0 MB Download SAVE_InCurv_xz_02.tif, file associated to 3D Image #304, 5.9 MB Download 06Jun22t02.mov, file associated to 3D Image #304, 537.0 MB Download Jun22t02sm.mov, file associated to 3D Image #304, 20.4 MB DownloadAdditional details
- DOI
- 10.22002/58eqf-fxn09
- TILT_SERIES_ID
- pdd2006-06-22-2
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
- Collected
-
2006-06-22
- Created
-
2008-06-18
- Updated
-
2014-08-11