MG1655 Sacculus

Description

Technical info

Tilt Series Date: 2007-08-11

Data Taken By: Lu Gan

Species / Specimen: Escherichia coli

Strain: MG1655 sacculi

Tilt Series Settings: Single Axis, tilt range: (-70.0°, 70.0°), step: 2.0°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -6.0 μm, magnification: 0x.

Acquisition Software: UCSF Tomo

Upload Method: rundir

Processing Software Used: imod

Collaborators and Roles: prepared by Lu Gan at Caltech; data collected by Lu Gan; processed by Lu Gan

Purification / Growth Conditions / Treatment: Grown to exponential phase in LB; Purified by hot SDS

Sample Preparation: C-flat 4/2, BSA treated 5nm gold, 100% humidity, -2mm offset,

Attached Files

MG1655_03.mrc, Tilt Series (Pixel Size 0.78 nm), 604.1 MB

MG1655_03.rec, Reconstruction (Pixel Size 0.78 nm), 597.3 MB