Published 2015 | Version v1
Dataset Open

nanochemowt_2ndday

  • 1. ROR icon California Institute of Technology

Description

This is my fourth attempt to grow Shewanella. This culture is wt grown at caltech in chemostat.Frozen here in vitrobot.

Technical info

Tilt Series Date: 2015-06-06

Data Taken By: Poorna Subramanian

Species / Specimen: Shewanella oneidensis

Strain: MR-1

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -8.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Moh and Sahand USC

Sample Preparation: This sample was frozen after being in oxygen deficiency for 40h. Checked by SEM and had nanowires. Vitrobot freezing conditions 6s blot force, 3s blot time, 1s drain time, 4ul on grid.

Attached Files

nanochemowt2day_atlas10023.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB

nanochemowt2day_atlas10023_full.rec, Reconstruction (Pixel Size 1.92 nm), 534.5 MB

Files

keyimg_psa2015-06-06-23.jpg

Files (4.0 GB)

Name Size
md5:e501d562d138af469ece0fa4b2053ebb
990.7 kB Preview Download
md5:f94d3109d2a72f416e7055545c0bc6e9
16.7 MB Preview Download
3.4 GB (external) Download
534.5 MB (external) Download

Additional details

Identifiers

DOI
10.22002/5h570-vqg02
TILT_SERIES_ID
psa2015-06-06-23

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2015-06-06
Created
2015-06-07
Updated
2015-06-23