Caltech Tomography Archive
Published 2013 | Version v1
Dataset Open

HUVECs infx pNLGFP

Creators

  • 1. ROR icon California Institute of Technology

Description

single tilt. constant angular increment, step: 1.0. tilt range: (-60, 60). dosage: 150/A2. defocus: -6um. magnification: 19000.

Technical info

Tilt Series Date: 2013-07-05

Data Taken By: Cora Woodward

Species / Specimen: HUVECs

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 19000x.

Microscope: Janelia Titan

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: UCSFTomo

Collaborators and Roles: pNLGFP obtained from Irvin Chen (UCLA)

Sample Preparation: Whole cells were grown on Au finder grids and infected with pNLGFP-VSVG. Sample frozen 24h post infection

Attached Files

13jul05_C161-2_HUVECspnl24hPI_montage_33x33_0026.mrc, Tilt Series (Pixel Size 0.56 nm), 3.3 GB

13jul05_C161-2_HUVECspnl24hPI_montage_33x33_0026_full.rec, Reconstruction (Pixel Size 2.24 nm), 602.8 MB

Files

keyimg_cwl2013-07-05-3.jpg

Files (4.0 GB)

Additional details

Identifiers

DOI
10.22002/5mwd7-qw329
TILT_SERIES_ID
cwl2013-07-05-3

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2013-07-05
Created
2013-07-06
Updated
2019-03-06