Legionella_delta-lspGHIJK
Contributors
Researchers:
Description
Technical info
Tilt Series Date: 2019-05-18
Data Taken By: Debnath Ghosal
Species / Specimen: Legionella pneumophila
Strain: Philadelphia-1_Lp02
Tilt Series Settings: Single Axis, tilt range: (-45.0°, 45.0°), step: 3.0°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -7.0 μm, magnification: 42000x.
Microscope: Janelia Titan
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain sent by Nicholas Cianciotto, Northwestern University. Data collected and processed by Debnath Ghosal.
Sample Preparation: 6 grids were frozen for this strain (2x concentrated culture, OD~4.8); Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.
Attached Files
DG8_201905180029.mrc, Tilt Series (Pixel Size 0.26 nm), 882.8 MB
DG8_201905180029_full.rec, Reconstruction (Pixel Size 1.04 nm), 445.4 MB
Files
keyimg_dga2019-05-18-59.jpg
Additional details
Identifiers
- DOI
- 10.22002/6256b-94x43
- TILT_SERIES_ID
- dga2019-05-18-59
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2019-05-18
- Created
-
2019-05-22
- Updated
-
2019-05-22