Published 2013 | Version v1
Dataset Open

Bacillus subtilis ponA mutant

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

ponA muntant of B. subtilis induced for competence

Technical info

Tilt Series Date: 2013-06-28

Data Taken By: Elitza Tocheva

Species / Specimen: Bacillus subtilis

Strain: MJ007, ponA null

Tilt Series Settings: Single Axis, tilt range: (-55.0°, 55.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: David Dubnau

Purification / Growth Conditions / Treatment: cells grown in competence medium

Sample Preparation: vitrobot

etl2013-06-28-7_slicer9908.jpg: inner budding membrane, Bacillus subtilis

Attached Files

bcomp0009.mrc, Tilt Series (Pixel Size 0.48 nm), 3.2 GB

bcomp0009_full.rec, Reconstruction (Pixel Size 1.92 nm), 801.8 MB

Files

etl2013-06-28-7_slicer9908.jpg

Files (4.0 GB)

Name Size
3.2 GB (external) Download
801.8 MB (external) Download
md5:9655b0dcdbe9896dc1b1b4cd99ae13d6
272.2 kB Preview Download
md5:38932284b17466409c7402c16ca4fb0c
1.0 MB Preview Download
md5:e7e1be2235c767677d65ba9a069b3e05
27.5 MB Preview Download

Additional details

Identifiers

DOI
10.22002/62jkb-zc492
TILT_SERIES_ID
etl2013-06-28-7

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2013-06-28
Created
2013-06-28
Updated
2014-08-09