O. tauri Propyzamide release
Description
Technical info
Tilt Series Date: 2010-02-12
Data Taken By: Lu Gan
Species / Specimen: Ostreococcus tauri
Strain: RCC745
Tilt Series Settings: Single Axis, tilt range: (-66.0°, 66.0°), step: 2.0°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -10.0 μm, magnification: 18000x.
Acquisition Software: UCSF tomo
Upload Method: rundir
Processing Software Used: imod
Collaborators and Roles: prepared by Lu Gan at Caltech; high-pressure frozen and cryosectioned by Mark Ladinsky; data collected by Lu Gan; processed by Lu Gan
Purification / Growth Conditions / Treatment: 9h in 1mM Hydroxyurea, 15h in 30uM Propyzamide, Washout Propyzamide
Sample Preparation: Cryoprotectant: 10mg/ml BSA, 2X 10nm gold, 20% Dextran; Cells mixed w/ cryoprotectant shortly before HPF
Attached Files
RCC745_RELtovis_022.mrc, Tilt Series (Pixel Size 1.26 nm), 771.8 MB
RCC745_RELtovis_022.rec, Reconstruction (Pixel Size 1.26 nm), 440.0 MB
Files
keyimg_lga2010-02-12-19.jpg
Additional details
Identifiers
- DOI
- 10.22002/63scb-2z040
- TILT_SERIES_ID
- lga2010-02-12-19
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2010-02-12
- Created
-
2010-05-12
- Updated
-
2010-05-12