Published 2015 | Version v1
Dataset Open

nanochemowt

  • 1. ROR icon California Institute of Technology

Description

This is my fourth attempt to grow Shewanella. This culture is wt grown at caltech in chemostat.Frozen here in vitrobot.

Technical info

Tilt Series Date: 2015-06-02

Data Taken By: Poorna Subramanian

Species / Specimen: Shewanella oneidensis

Strain: MR-1

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -7.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Moh and Sahand USC

Sample Preparation: This sample was frozen after being in oxygen deficiency for 16h. Checked by SEM and had nanowires. Vitrobot freezing conditions 6s blot force, 3s blot time, 1s drain time, 4ul on grid.

Attached Files

nanochemo_atlas10034.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB

nanochemo_atlas10034_full.rec, Reconstruction (Pixel Size 1.92 nm), 534.5 MB

Files

keyimg_psa2015-06-02-26.jpg

Files (4.0 GB)

Name Size
md5:5bbf135efddcd6c0b1ddd31c08150778
966.5 kB Preview Download
md5:da3d0b88680d85480f67cebb64c8e312
17.2 MB Preview Download
3.4 GB (external) Download
534.5 MB (external) Download

Additional details

Identifiers

DOI
10.22002/64cv4-gn623
TILT_SERIES_ID
psa2015-06-02-26

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2015-06-02
Created
2015-06-02
Updated
2015-06-07