Published 2016 | Version v1
Dataset Open

HeLa cells expressing TRIM5α-YFP

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

4 hours incubation MG-132 (0.4 ul) 1 μg/ml.

Technical info

Tilt Series Date: 2016-05-05

Data Taken By: Stephen Carter

Species / Specimen: Hela cells expressing TRIM5a-YFP

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -10.0 μm, magnification: 18000x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: reupload_downloaded

Processing Software Used: IMOD

Collaborators and Roles: Tom Hope

Purification / Growth Conditions / Treatment: Grown on R2/2 London finder grids coated with fibronectin

Sample Preparation: Manual Blotting

Attached Files

tomography7.mrc, Tilt Series (Pixel Size 6.167 nm), 2.8 GB

tomography7.rec, Reconstruction (Pixel Size 24.668 nm), 1.8 GB

tomography7join_full.rec, Reconstruction (Pixel Size 0.0 nm), 1.1 GB

Files

keyimg_scd2016-05-05-7.jpg

Files (5.7 GB)

Name Size
md5:9a150e0ef51bb2e5e5b2ce5705a625f6
1.1 MB Preview Download
md5:5fcf080f13b438e8b5dcfc78dd81c98c
17.0 MB Preview Download
2.8 GB (external) Download
1.8 GB (external) Download
1.1 GB (external) Download

Additional details

Identifiers

DOI
10.22002/6856m-1md34
TILT_SERIES_ID
scd2016-05-05-7

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2016-05-05
Created
2021-03-12
Updated
2021-03-30