Vibrio cholerae

Description

Technical info

Tilt Series Date: 2015-10-01

Data Taken By: Ariane Briegel

Species / Specimen: Vibrio cholerae

Strain: PM22, lacZ- Δvc1397 Δvc1402

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -8.0 μm, magnification: 34000x.

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Simon Ringgaard

Purification / Growth Conditions / Treatment: Briegel, A., Ortega, D. R., Mann, P., Kjaer, A., Ringgaard, S. and Jensen, G. J. (2016). Chemotaxis cluster 1 proteins form cytoplasmic arrays in Vibrio cholerae and are stabilized by a double signaling domain receptor DosM. Proc Natl Acad Sci U S A 113, 10412-10417.

aba2015-10-01-31_slicer16189.jpg: The spacing between fibers in this image seems to be about 5.25 +/-0.3 nm. . It might be DNA. . (If I find the paper where I saw something similar, I'll post the link.) . Andrew . (just testing grab-n-note) aba2015-10-01-31_slicer16186.jpg: crazy structure in lysed cell aba2015-10-01-31_slicer16185.jpg: crazy structure in lysed cell

Attached Files

PM22deltaCheA1trippleW0033_full.rec, Reconstruction (Pixel Size 1.28 nm), 712.7 MB

PM22deltaCheA1trippleW0033.mrc, Tilt Series (Pixel Size 0.32 nm), 3.4 GB