Legionella IcmQ71::TevS 30C_Off

Description

Technical info

Tilt Series Date: 2019-05-28

Data Taken By: Debnath Ghosal

Species / Specimen: Legionella pneumophila

Strain: Philadelphia-1_Lp02

Tilt Series Settings: Single Axis, tilt range: (-51.0°, 51.0°), step: 3.0°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -6.0 μm, magnification: 42000x.

Microscope: Janelia Titan

Acquisition Software: Undefined

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain sent to us by Howard Shuman (University of Chicago). Data collected by Debnath Ghosal and Yuxi Liu.

Sample Preparation: Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.

Attached Files

HS004G-OFF-tomo0001.mrc, Tilt Series (Pixel Size 0.26 nm), 996.7 MB

HS004G-OFF-tomo0001_part35_6.rec, Reconstruction (Pixel Size 1.04 nm), 445.4 MB