Myxococcus xanthus PilP-sfGFP

Description

Technical info

Tilt Series Date: 2014-03-08

Data Taken By: Yi-Wei Chang

Species / Specimen: Myxococcus xanthus

Strain:

Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -8.0 μm, magnification: 26000x.

Microscope: Janelia Titan

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain form Lotte Sogaard-Andersen

Purification / Growth Conditions / Treatment: Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.8

Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.

ycw2014-03-08-21_slicer3779.jpg: Myxococcus xanthus. Another example of vesicles in a row. ycw2014-03-08-21_slicer7949.jpg: filament bundle, Myxococcus xanthus ycw2014-03-08-21_slicer7951.jpg: Myxococcus xanthus, T6SS ycw2014-03-08-21_slicer7953.jpg: unknown

Attached Files

20140308_YWC093_10034.mrc, Tilt Series (Pixel Size 0.42 nm), 3.6 GB

20140308_YWC093_10034_full.rec, Reconstruction (Pixel Size 1.68 nm), 688.9 MB