Myxococcus xanthus pilM-YFP
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2014-05-14
Data Taken By: Yi-Wei Chang
Species / Specimen: Myxococcus xanthus
Strain:
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -8.0 μm, magnification: 26000x.
Microscope: Janelia Titan
Acquisition Software: Undefined
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain form Lotte Sogaard-Andersen
Purification / Growth Conditions / Treatment: Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.9
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated 10 nm gold. -4 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.
ycw2014-05-14-3_slicer5135.jpg: Pilli ycw2014-05-14-3_slicer5137.jpg: Unknown
Attached Files
20140514_Janelia_YWC096_10003_part131_10.rec, Reconstruction (Pixel Size 1.32 nm), 688.9 MB
20140514_Janelia_YWC096_10003.mrc, Tilt Series (Pixel Size 0.33 nm), 3.6 GB
Files
keyimg_ycw2014-05-14-3.jpg
Files
(4.3 GB)
| Name | Size | |
|---|---|---|
| 3.6 GB | (external) Download | |
| 688.9 MB | (external) Download | |
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md5:624524a317f73fe5b27986c9f012b5e3
|
1.0 MB | Preview Download |
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md5:46862cc62bd3d9d4bfd67241bd571e5c
|
23.1 MB | Preview Download |
|
md5:81df26be3d38a8868f36e86c48188c9d
|
387.3 kB | Preview Download |
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md5:dc50fbf3ce73a6abbe61036242d3d77e
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365.7 kB | Preview Download |
Additional details
Identifiers
- DOI
- 10.22002/6sp9z-hyz43
- TILT_SERIES_ID
- ycw2014-05-14-3
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2014-05-14
- Created
-
2014-05-15
- Updated
-
2014-07-08