Published 2017 | Version v1
Dataset Open

Staphylococcus aureus_WT

  • 1. ROR icon California Institute of Technology

Description

T7SS

Technical info

Tilt Series Date: 2017-06-29

Data Taken By: Debnath Ghosal

Species / Specimen: Unspecified

Strain: None

Tilt Series Settings: Single Axis, tilt range: (-50.0°, 50.0°), step: 1.5°, constant angular increment, dosage: 280.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain sent to us by Tracy Palmer, University of Dundee. Data collected and processed by Ki Woo and Debnath Ghosal.

Sample Preparation: Media: TSA 500g (Oxoid TSA and TSB from Thermo Fisher. 10525943), TBS 500g (10137562)Dilute an overnight culture 1/100Incubate it for 4 hr (--> OD 2)Vitrobot

Attached Files

Saureus_WT_06290024.mrc, Tilt Series (Pixel Size 0.39 nm), 1.9 GB

Saureus_WT_06290024_full.rec, Reconstruction (Pixel Size 1.56 nm), 445.4 MB

Files

keyimg_dga2017-06-29-35.jpg

Files (2.4 GB)

Name Size
md5:181a5fa42644486e650e07484c6b9bfa
1.1 MB Preview Download
md5:6984436e3e618c3386ef0d4fd37cc7f6
14.6 MB Preview Download
1.9 GB (external) Download
445.4 MB (external) Download

Additional details

Identifiers

DOI
10.22002/6v16z-mjw60
TILT_SERIES_ID
dga2017-06-29-35

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2017-06-29
Created
2017-07-03
Updated
2017-07-05