Published 2014 | Version v1
Dataset Open

HeLa cells

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

Looking for ESCRT around midbodies

Technical info

Tilt Series Date: 2014-11-26

Data Taken By: Rasika Ramdasi

Species / Specimen: HeLa cells

Strain: ATCC

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -10.0 μm, magnification: 18000x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: UCSF tomo

Collaborators and Roles: Cells provided by Doug Mckarty

Sample Preparation: HeLa cells+ siRNA nup153+Thymidine 2mM for 14 h.

Attached Files

midbody10012.mrc, Tilt Series (Pixel Size 0.6 nm), 3.4 GB

midbody10012_full.rec, Reconstruction (Pixel Size 2.4 nm), 623.6 MB

Files

keyimg_rra2014-11-26-28.jpg

Files (4.1 GB)

Name Size
md5:fd82e5e7bf3bcbab70a08c0d914c7da6
1.0 MB Preview Download
md5:23dd2de53b27c7786d117f937a9e4f5b
15.9 MB Preview Download
3.4 GB (external) Download
623.6 MB (external) Download

Additional details

Identifiers

DOI
10.22002/6vc47-1q190
TILT_SERIES_ID
rra2014-11-26-28

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2014-11-26
Created
2014-11-27
Updated
2014-12-01