Calreticulin-YFP
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2014-09-09
Data Taken By: Stephen Carter
Species / Specimen: INS-1E Pancreatic Cells expressing Calreticulin-YFP
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -15.0 μm, magnification: 18000x.
Microscope: Caltech Polara
Acquisition Software: Undefined
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Zach Freidberg
Purification / Growth Conditions / Treatment: INS-1E cells grown on gold finder grids and plunge frozen using the vitrobot III blotted both sides pressure setting 1. Grids coated with fibronectin and 200 nm red fluorescent beads F8763 added to grids before freezing.
Attached Files
tomography9.mrc, Tilt Series (Pixel Size 0.6 nm), 3.4 GB
tomography9_part121_1.rec, Reconstruction (Pixel Size 2.4 nm), 1.1 GB
Files
keyimg_scd2014-09-09-8.jpg
Additional details
Identifiers
- DOI
- 10.22002/6wh8t-vjs81
- TILT_SERIES_ID
- scd2014-09-09-8
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2014-09-09
- Created
-
2014-09-09
- Updated
-
2015-05-06