Hyphomonas "AR43"
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2012-03-04
Data Taken By: Yi-Wei Chang
Species / Specimen: Hyphomonas neptunium
Strain: ATCC 15444
Tilt Series Settings: Single Axis, tilt range: (-50.0°, 50.0°), step: 1.0°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -10.0 μm, magnification: 34000x.
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain from Martin Thanbichler
Purification / Growth Conditions / Treatment: Cells grown overnight in Marine Broth (MB) medium at 30 degrees. Expression of Venus labeled HNK0708 was induced by adding final 300 uM CuCl2 and incubated at 30 degrees for 24 hr.
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix.
ycw2012-03-04-26_slicer8734.jpg: Hyphomonas neptunium . ribosome exclusion zone
Attached Files
2012Mar04_AR43_24hr_30020.mrc, Tilt Series (Pixel Size 0.644 nm), 842.5 MB
2012Mar04_AR43_24hr_30020_full.rec, Reconstruction (Pixel Size 1.288 nm), 516.1 MB
Files
keyimg_ycw2012-03-04-26.jpg
Additional details
Identifiers
- DOI
- 10.22002/6zvy1-tmg35
- TILT_SERIES_ID
- ycw2012-03-04-26
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2012-03-04
- Created
-
2012-03-05
- Updated
-
2014-07-28