HUVECs infx pNLGFP
Description
Technical info
Tilt Series Date: 2013-11-08
Data Taken By: Cora Woodward
Species / Specimen: HUVECs
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 170.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x.
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: UCSFTomo
Collaborators and Roles: pNLGFP obtained from Irvin Chen (UCLA)
Purification / Growth Conditions / Treatment: Whole cells were grown on Au finder grids and infected with pNLGFP- VSVG. Sample frozen 24h post infection
Sample Preparation: manual blotting
Attached Files
13nov08_C176-2b_pNLgfpHUVECs_ATb1_0004.mrc, Tilt Series (Pixel Size 0.49 nm), 3.4 GB
13nov08_C176-2b_pNLgfpHUVECs_ATb1_0004_full.rec, Reconstruction (Pixel Size 1.96 nm), 445.4 MB
Files
keyimg_cwl2013-11-08-4.jpg
Additional details
Identifiers
- DOI
- 10.22002/71eyg-82a90
- TILT_SERIES_ID
- cwl2013-11-08-4
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2013-11-08
- Created
-
2013-11-15
- Updated
-
2019-03-07