Myxococcus xanthus pilM-YFP
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2014-05-14
Data Taken By: Yi-Wei Chang
Species / Specimen: Myxococcus xanthus
Strain:
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -8.0 μm, magnification: 26000x.
Microscope: Janelia Titan
Acquisition Software: Undefined
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain form Lotte Sogaard-Andersen
Purification / Growth Conditions / Treatment: Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.9
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated 10 nm gold. -4 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.
ycw2014-05-14-5_slicer5149.jpg: Budding Vesicle ycw2014-05-14-5_slicer5152.jpg: Flat Vesicle ycw2014-05-14-5_slicer9049.jpg: flat vesicle . Myxococcus xanthus . snapshot by Megan
Attached Files
20140514_Janelia_YWC096_10005_part131_2.rec, Reconstruction (Pixel Size 1.32 nm), 688.9 MB
20140514_Janelia_YWC096_10005.mrc, Tilt Series (Pixel Size 0.33 nm), 3.6 GB
Files
keyimg_ycw2014-05-14-5.jpg
Files
(4.3 GB)
| Name | Size | |
|---|---|---|
| 3.6 GB | (external) Download | |
| 688.9 MB | (external) Download | |
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md5:79f7d8c5b01a31ff7bc9651706c75387
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982.2 kB | Preview Download |
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md5:85d840e229396a3d5d0295a320ea417b
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27.1 MB | Preview Download |
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md5:3af956d08b5ebb096c7f6f42a95b7906
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351.7 kB | Preview Download |
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md5:ef30f1d034a13ef14f70e2164e9b6880
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346.0 kB | Preview Download |
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md5:6390c34733df56241aa2cc2a36bbfbb6
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91.5 kB | Preview Download |
Additional details
Identifiers
- DOI
- 10.22002/71j4c-ang43
- TILT_SERIES_ID
- ycw2014-05-14-5
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2014-05-14
- Created
-
2014-05-15
- Updated
-
2014-07-30