AimB depletion control w/ xylose
Description
Technical info
Tilt Series Date: 2009-09-21
Data Taken By: Zhuo Li
Species / Specimen: Caulobacter crescentus
Strain: NA1000
Tilt Series Settings: Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 110.0 eV/Ų, defocus: -16.0 μm, magnification: 18000x.
Acquisition Software: Leginon
Upload Method: rundir
Processing Software Used: RAPTOR, Peach, IMOD
Collaborators and Roles: The AimB_depletion strain was constructed by John Werner, data collected by ZL
Purification / Growth Conditions / Treatment: AimB depletion is achieved by serial exchanging media from that including 0.02% xylose to that including 0.3% glucose.
Sample Preparation: quantifoil grid R2/1 or R2/2, 10nm gold, 100% humidity
zla2009-09-21-18_slicer10167.jpg: Caulobacter crescentus . flat vesicle zla2009-09-21-18_slicer10170.jpg: Caulobacter crescentus . flat vesicle zla2009-09-21-18_slicer10171.jpg: Caulobacter crescentus . flat vesicle zla2009-09-21-18_slicer10172.jpg: Caulobacter crescentus . filament
Attached Files
09sep21a_AimBxyl_036.mrc, Tilt Series (Pixel Size 1.192 nm), 536.9 MB
09sep21a_AimBxyl_036_full.rec, Reconstruction (Pixel Size 2.384 nm), 471.9 MB
Files
keyimg_zla2009-09-21-18.jpg
Files
(1.0 GB)
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Additional details
Identifiers
- DOI
- 10.22002/71mtq-zxd69
- TILT_SERIES_ID
- zla2009-09-21-18
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2009-09-21
- Created
-
2010-04-01
- Updated
-
2014-08-12