Published 2014 | Version v1
Dataset Open

E. Coli delta53

Creators

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

Mutant MreB with sfGFP in sandwich position. WT MreB is replaced at the original locus with this fusion.

Technical info

Tilt Series Date: 2014-04-01

Data Taken By: Matt Swulius

Species / Specimen: Escherichia Coli

Strain: delta53

Tilt Series Settings: Single Axis, tilt range: (-64.0°, 64.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 26000x.

Microscope: Janelia Titan

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: raptor

Collaborators and Roles: Josh Schaevitz

Purification / Growth Conditions / Treatment: 37 celsius to OD 600 ~ 0.3

Sample Preparation: Blotted manually from rear in vitrobot at 100% humidity

Attached Files

040114_delta53_0018.mrc, Tilt Series (Pixel Size 0.42 nm), 3.6 GB

040114_delta53_0018_part131_4.rec, Reconstruction (Pixel Size 1.68 nm), 861.2 MB

Files

keyimg_mst2014-04-01-45.jpg

Files (4.5 GB)

Name Size
3.6 GB (external) Download
861.2 MB (external) Download
md5:7b030a2cc02f1a7e1f89ec8c15d642ff
1.0 MB Preview Download
md5:4b9f1b0e61135de1185781a2035fb842
36.6 MB Preview Download

Additional details

Identifiers

DOI
10.22002/73ww0-0e292
TILT_SERIES_ID
mst2014-04-01-45

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2014-04-01
Created
2014-04-14
Updated
2014-04-15