Helicobacter pylori 26695

Description

Technical info

Tilt Series Date: 2013-10-25

Data Taken By: Yi-Wei Chang

Species / Specimen: Helicobacter pylori

Strain: 26695

Tilt Series Settings: Single Axis, tilt range: (-50.0°, 50.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 26000x.

Microscope: Janelia Titan

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Cell prepared by Lee Rettberg.

Purification / Growth Conditions / Treatment: Cells grown in BLBB + 10% heat inactivated fetal bovine serum at 37 degrees + 5% CO2 for 4 days, OD600=0.4

Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu R2/2 200

ycw2013-10-25-69_slicer10169.jpg: Helicobacter pylori . inclusion body . chemoreceptor . Ribosome exclusion zone ycw2013-10-25-69_slicer10174.jpg: Helicobacter pylori . filament . flat vesicle ycw2013-10-25-69_slicer17919.jpg: cag T4SS wi fi particle ycw2013-10-25-69_slicer17920.jpg: membrane vesicle chains?

Attached Files

20131025_Janelia_YWC043_20015.mrc, Tilt Series (Pixel Size 0.42 nm), 2.8 GB

20131025_Janelia_YWC043_20015_full.rec, Reconstruction (Pixel Size 1.68 nm), 688.9 MB