Myxococcus xanthus delta-tsaP
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2014-07-07
Data Taken By: Yi-Wei Chang
Species / Specimen: Myxococcus xanthus
Strain:
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -8.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain form Lotte Sogaard-Andersen
Purification / Growth Conditions / Treatment: Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Manual blot, ~3 sec blot time. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder.
ycw2014-07-07-28_slicer7437.jpg: nested vesicles in Myxococcus xanthus delta-tsaP . extracellular vesicles ycw2014-07-07-28_slicer10399.jpg: nested vesicles . Myxococcus xanthus . snapshot by Megan
Attached Files
20140707_YWC106_10030.mrc, Tilt Series (Pixel Size 0.39 nm), 3.7 GB
20140707_YWC106_10030_full.rec, Reconstruction (Pixel Size 1.56 nm), 712.7 MB
Files
keyimg_ycw2014-07-07-28.jpg
Files
(4.5 GB)
| Name | Size | |
|---|---|---|
| 3.7 GB | (external) Download | |
| 712.7 MB | (external) Download | |
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md5:a1daebc3623cc6cb9ec511e743f77218
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1.0 MB | Preview Download |
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md5:6fe33c43d1a4371a4a83e9d26f78beb7
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22.9 MB | Preview Download |
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md5:6f12869aed2d0080f51ecebdfac2717d
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73.9 kB | Preview Download |
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md5:14ec721c43a4075fb748567f310c999f
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423.8 kB | Preview Download |
Additional details
Identifiers
- DOI
- 10.22002/78t9k-yqv04
- TILT_SERIES_ID
- ycw2014-07-07-28
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2014-07-07
- Created
-
2014-07-08
- Updated
-
2014-08-14