Published 2012
| Version v1
Dataset
Open
Cryo-sectioned synchronized Jurkat
Contributors
Researcher:
Description
Jurkats were cell-cycle synchronized (Sarah Cheng) using nocodazole then HP frozen and cryo-sectioned by Mark Ladinsky-Grid4
Technical info
Tilt Series Date: 2012-08-16
Data Taken By: Cora Woodward
Species / Specimen: Jurkat Human T-cell
Strain: Clone E6-1
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x.
Acquisition Software: UCSF tomo
Upload Method: rundir
Processing Software Used: imod
Collaborators and Roles: Cryo-sectioning by Mark Ladinsky.
Purification / Growth Conditions / Treatment: nocodazole synchronized
Sample Preparation: cryo-sectioned by Mark Ladinsky
Attached Files
12aug16_mitoticjurkat_BINNED_9_full.rec, Reconstruction (Pixel Size 2.0 nm), 424.0 MB
12aug16_mitoticjurkat_9.mrc, Tilt Series (Pixel Size 0.5 nm), 2.3 GB
Files
keyimg_cwl2012-08-16-8.jpg
Additional details
Identifiers
- DOI
- 10.22002/78yp0-d1m66
- TILT_SERIES_ID
- cwl2012-08-16-8
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2012-08-16
- Created
-
2015-09-16
- Updated
-
2015-09-16