Caltech Tomography Archive
Published 2012 | Version v1
Dataset Open

Cryo-sectioned synchronized Jurkat

Woodward, Cora1
  • 1. ROR icon California Institute of Technology
Researcher:
Ladinsky, Mark

Description

Jurkats were cell-cycle synchronized (Sarah Cheng) using nocodazole then HP frozen and cryo-sectioned by Mark Ladinsky-Grid4

Files

keyimg_cwl2012-08-16-8.jpg
Files (9.3 MB)
Name Size
keyimg_cwl2012-08-16-8.jpg
md5:5aabc58ffd64dc02337048e10e14020e
913.3 kB Preview Download
keymov_cwl2012-08-16-8.mp4
md5:180273969e9b7f0094b39de8728f7faf
8.4 MB Download

Technical info

Tilt Series Date: 2012-08-16

Data Taken By: Cora Woodward

Species / Specimen: Jurkat Human T-cell

Strain: Clone E6-1

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x.

Acquisition Software: UCSF tomo

Upload Method: rundir

Processing Software Used: imod

Collaborators and Roles: Cryo-sectioning by Mark Ladinsky.

Purification / Growth Conditions / Treatment: nocodazole synchronized

Sample Preparation: cryo-sectioned by Mark Ladinsky

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-08-16-8/3dimage_68666/

12aug16_mitoticjurkat_BINNED_9_full.rec, Reconstruction (Pixel Size 2.0 nm), 424.0 MB Download

12aug16_mitoticjurkat_9.mrc, Tilt Series (Pixel Size 0.5 nm), 2.3 GB Download

Additional details

Identifiers Funding Dates
Created:
May 23, 2023
Modified:
August 26, 2025