AD348_delVirB_Agro

Description

Technical info

Tilt Series Date: 2016-01-06

Data Taken By: Debnath Ghosal

Species / Specimen: Agrobacterium tumefaciens

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -8.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain constructed by Anath Das. Data collected and processed by Debnath Ghosal using Caltech tomography pipeline.

Purification / Growth Conditions / Treatment: Grow at 19C for 4 days on induction plate. Add 400 ul of induction media to one of the induction plates (containing lawn of Agrobacterium AD348_delVirB) and wash the plate by gently rolling the liquid. Add gold to this: 1ul of gold to 4 ul of cells, freeze.

Sample Preparation: Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.

dga2016-01-06-14_slicer18047.jpg: V-shaped membrane invagination in Agrobacterium tumefaciens dga2016-01-06-14_slicer18048.jpg: Extracellular polyphosphate bodies from the cytoplasm

Attached Files

AD348_delVirB0014.rec, Reconstruction (Pixel Size 19.2 nm), 890.9 MB

AD348_delVirB0014.mrc, Tilt Series (Pixel Size 4.8 nm), 3.4 GB