Published 2017
| Version v1
Dataset
Open
S. aureus RN6390 ΔESX
- Researchers:
- Palmer, Tracy
- Chreifi, Georges
Description
Staphylococcus aureus strain RN6390 ΔESX deletion mutant
Files
keyimg_gca2017-09-28-85.jpg
Technical info
Tilt Series Date: 2017-09-28
Data Taken By: Georges Chreifi Species / Specimen: Staphylococcus aureus Strain: subsp. aureus str. Newman Tilt Series Settings: Single Axis, tilt range: (-56.0°, 44.0°), step: 2°, constant angular increment, dosage: 265.0 eV/Ų, defocus: -8.0 μm, magnification: 27500x. Microscope: Caltech Polara Acquisition Software: Undefined Upload Method: pipeline Processing Software Used: etomo + manual tracking Collaborators and Roles: Strain provided by Tracy Palmer at the University of Dundee. Data collected and processed by Georges Chreifi Purification / Growth Conditions / Treatment: Cells were grown in Tryptic Soy Broth (TSB) liquid culture overnight with no supplements. The overnight culture was then used to inoculate fresh TSB, grown until an OD of ~2, and plunge-frozen. Sample Preparation: 4 grids were frozen. Vitrobot (Mark IV) settings: 95% humidity, 2µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200 mesh. Added 1ul of gold to 4 ul of cells prior to freezing.External Files
Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/gca2017-09-28-85/rawdata/
atlas_30032.mrc, Tilt Series (Pixel Size 0.39 nm), 1.5 GB Download atlas_30032_full.rec, Reconstruction (Pixel Size 1.56 nm), 445.4 MB Download atlas_30032_full_1k.rec, Reconstruction (Pixel Size 1.615 nm), 534.5 MB DownloadAdditional details
- DOI
- 10.22002/7f44a-trt17
- TILT_SERIES_ID
- gca2017-09-28-85
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
- Collected
-
2017-09-28
- Created
-
2017-10-02
- Updated
-
2017-11-08