Proteus
Contributors
Researchers:
Description
Technical info
Tilt Series Date: 2015-11-19
Data Taken By: Qing Yao
Species / Specimen: Proteus mirabilis
Strain: HI4320
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -10.0 μm, magnification: 33599x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: IMOD
Collaborators and Roles: Douglas B. Weibel lab, Wisconsin-Madison university. Preparethe cell and design the research.
Purification / Growth Conditions / Treatment: This sample is prepared with the help of George Aucer, Ph. D student at Douglas B. Weibel lab, Wisconsin-Madison university
Sample Preparation: OD=6, 4 ul loading. bloting condition 6s force/ 3s time/ 1s dry time, QUANTIFOIL® – Holey Carbon Films 200 mesh
Attached Files
atlas20001_full.rec, Reconstruction (Pixel Size 1.28 nm), 445.4 MB
atlas20001.mrc, Tilt Series (Pixel Size 0.32 nm), 3.4 GB
Files
keyimg_qya2015-11-19-25.jpg
Additional details
Identifiers
- DOI
- 10.22002/7h9jm-ytc18
- TILT_SERIES_ID
- qya2015-11-19-25
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2015-11-19
- Created
-
2015-11-21
- Updated
-
2018-08-03