Caulobacter crescentus

Description

Technical info

Tilt Series Date: 2004-02-16

Data Taken By: Prabha Dias

Species / Specimen: Caulobacter crescentus

Strain: NA1000

Tilt Series Settings: Dual Axis, tilt range: (-62.0°, 62.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -2.0 μm, magnification: 0x.

Acquisition Software:

Upload Method: rundir

Processing Software Used: imod, bsoft

Collaborators and Roles: specimen prep &data collection: D. Prabha Dias (not processed)

Purification / Growth Conditions / Treatment: ATCC media (made by A.Frangakis); 0.5ml culture in perforated 1.5ml microfuge tube; extended stationary phase; see DPD notebook Jan 14-16, 2004

Sample Preparation: box 79, grid 3; Lacy carbon; 10nm gold on grids & in cell suspension; 6 ul per grid; offset -3, blot 1 sec, no drain time; see DPD notebook Jan 14-16, 2004

Attached Files

CC_A2_60e_axis1.mrc, Tilt Series (Pixel Size 1.2 nm), 1.1 GB

CC_A2_60e_axis2.mrc, Tilt Series (Pixel Size 1.2 nm), 1.1 GB