#92+E.coli bamA purified
Description
Technical info
Tilt Series Date: 2015-04-30
Data Taken By: Poorna Subramanian
Species / Specimen: Escherichia coli
Strain:
Tilt Series Settings: Single Axis, tilt range: (-66.0°, 66.0°), step: 2.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -10.0 μm, magnification: 33000x.
Microscope: Janelia Titan
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Chris Hayes and David Low UCSB
Sample Preparation: These are E.cloacae mini cells (#92) mixed with purified E.coli BamA protein receptor. Both cells and BamA were blocked with BSA- 100g/ml for 30 minutes before mixing for 20 minutes.
Attached Files
PS96A-atlas0009.mrc, Tilt Series (Pixel Size 0.33 nm), 1.8 GB
PS96A-atlas0009_part67_3.rec, Reconstruction (Pixel Size 1.32 nm), 430.6 MB
Files
keyimg_psa2015-04-30-10.jpg
Additional details
Identifiers
- DOI
- 10.22002/7hxa8-9eb20
- TILT_SERIES_ID
- psa2015-04-30-10
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2015-04-30
- Created
-
2015-05-01
- Updated
-
2017-01-12