Caltech Tomography Archive
Published 2017 | Version v1
Dataset Open

Halobacterium salinarum

Ghosal, Debnath1
  • 1. ROR icon California Institute of Technology

Description

Archaella and plate

Files

keyimg_dga2017-05-26-13.jpg
Files (15.8 MB)
Name Size
keymov_dga2017-05-26-13.mp4
md5:279376a65fcbb5a7c52a3177c2282a3e
14.7 MB Download
keyimg_dga2017-05-26-13.jpg
md5:2622520bcfad2cff45bf70c7b0678521
1.1 MB Preview Download

Technical info

Tilt Series Date: 2017-05-26

Data Taken By: Debnath Ghosal

Species / Specimen: Halobacterium salinarum

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 110.0 eV/Ų, defocus: -7.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain sent to us by Robert Gunsalus, UCLA. Data collected and processed by Ki Woo and Debnath Ghosal

Purification / Growth Conditions / Treatment: Standard H. salinarum medium

Sample Preparation: Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3.5 sec. Wait time 8 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-05-26-13/rawdata/

Halobacterium_20170525d0005.mrc, Tilt Series (Pixel Size 0.39 nm), 1.7 GB Download

Halobacterium_20170525d0005_part61_4.rec, Reconstruction (Pixel Size 1.56 nm), 445.4 MB Download

Additional details

Identifiers Funding Dates
Created:
May 25, 2023
Modified:
August 27, 2025