Hippocampal Neurons (DIV 7)
Contributors
Researchers:
Description
Technical info
Tilt Series Date: 2015-05-03
Data Taken By: Matt Swulius
Species / Specimen: Rat Hippocampal Neuron (cultured)
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Xiawei Zhuang, Ruobo Zhou (cultured neurons and STORM imaging)
Purification / Growth Conditions / Treatment: Plunge frozen at DIV 7. Grids were treated with PDL prior to plating and 3 ul concentrated gold suspended in growth media was added to grid before freezing.
Sample Preparation: Grids were plunge froze in a vitrobot at 95 % humidity adn 22 C. They were manually blotted from behind.
Attached Files
050315_hipp_neuron_7div_grid3_hole2_tomo0003.mrc, Tilt Series (Pixel Size 0.41 nm), 3.4 GB
050315_hipp_neuron_7div_grid3_hole2_tomo0003_full.rec, Reconstruction (Pixel Size 1.64 nm), 445.4 MB
Files
keyimg_mst2015-05-03-2.jpg
Additional details
Identifiers
- DOI
- 10.22002/7xh6a-6w152
- TILT_SERIES_ID
- mst2015-05-03-2
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2015-05-03
- Created
-
2015-05-05
- Updated
-
2015-05-07