Published 2010
| Version v1
Dataset
Open
M259Q chemoreceptor mutant
Contributors
Researcher:
Description
M259Q HAMP mutant locked in 'CheA activating state'
Technical info
Tilt Series Date: 2010-03-21
Data Taken By: Ariane Briegel
Species / Specimen: Escherichia coli
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 34000x.
Acquisition Software: Leginon
Upload Method: webload
Processing Software Used: IMOD
Collaborators and Roles: John Parkinson
Purification / Growth Conditions / Treatment: grown in TB medium with Chloramphenicol, induced with salicylate (0.6 µM) for three hours, then penicillin treated for one hour
Attached Files
10mar21a_EcoliM259Q_003.st, Tilt Series (Pixel Size 0.632 nm), 1.0 GB
10mar21a_EcoliM259Q_003.rec, Reconstruction (Pixel Size 0.632 nm), 220.2 MB
Files
keyimg_aba2010-03-21-3.jpg
Additional details
Identifiers
- DOI
- 10.22002/7zy4e-hhk83
- TILT_SERIES_ID
- aba2010-03-21-3
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2010-03-21
- Created
-
2010-07-12
- Updated
-
2010-07-12