Published 2006
| Version v1
Dataset
Open
Wild type NA1000
Description
This is the cell shown in Fig. 2(E-E3) and Fig. 5 of Li et al. 2007.Keywords: Division site. FtsZ filaments.
Files
Fig3a22wtamira.jpg
Files
(17.3 MB)
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Technical info
Tilt Series Date: 2006-06-07
Data Taken By: Zhuo Li Species / Specimen: Caulobacter crescentus Strain: NA1000 Tilt Series Settings: Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Ų, defocus: -12.0 μm, magnification: 0x. Acquisition Software: UCSF Tomo Upload Method: webload Processing Software Used: IMOD, Amira Collaborators and Roles: Cells were cultured by Zhuo Li. Data were collected and processed by Zhuo Li. Purification / Growth Conditions / Treatment: NA1000 cells were grown at 30 C in PYE until they reached log phase. Swarmer cells were then isolated by centrifugation method, resuspended in M2G minimal medium, and allowed to progress through the cell cycle. 10 ug/ml A22 was added to depolymerize MreB filaments. Sample Preparation: 4 ul of cell culture at different time points were mixed with 10nm colloidal gold and plunge-frozen onto glow-discharged Quantifoil R2/2, R2/1, or R1.2/1.3 grids using a Vitrobot (FEI). Fig3a22wtamira.jpg: Segmentation pg_1.jpg: Segmentation pg_closrup.jpg: SegmentationExternal Files
Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/zla2006-06-07-2/3dimage_693/
a22wt690660w1d12u454c03_fixed_bin.mrc.rec.mrc, Reconstruction (Pixel Size 1.34 nm), 179.7 MB Download da22wt690660w1d12u454c03_fixed_bin.mrc.rec_0015.mrc, Denoised subtomogram, 179.7 MB Download a22wt690660w1d12u454c03.mrc, Tilt Series (Pixel Size 0.67 nm), 1.0 GB Download a22wt690660w1d12u454c03_fixed_bin.mrc_full.rec, Reconstruction (Pixel Size 1.34 nm), 943.7 MB Download da22wt690660w1d12u454c03trim.am, Segmentation, 49.3 MB Download da22wt690660w1d12u454c03trim-labels.am, Segmentation, 3.0 MB Download da22wt690660w1d12u454c03trim-labels1.am, Segmentation, 2.5 MB Download da22wt690660w1d12u454c03trim-labelsFL.am, Segmentation, 779.7 kB Download da22wt690660w1d12u454c03trim-labelsFL.surf, Segmentation, 261.1 kB Download da22wt690660w1d12u454c03trim-labels_IM.am, Segmentation, 1.2 MB Download da22wt690660w1d12u454c03trim-labels_IM.surf, Segmentation, 21.0 MB Download da22wt690660w1d12u454c03trim-labels_IM1.am, Segmentation, 1.1 MB Download da22wt690660w1d12u454c03trim-labelsOM.am, Segmentation, 1.2 MB Download da22wt690660w1d12u454c03trim-labelsOM.surf, Segmentation, 24.1 MB Download da22wt690660w1d12u454c03trim-labelsOM_SLay.am, Segmentation, 2.2 MB Download da22wt690660w1d12u454c03trim-labelsOUTlayer.am, Segmentation, 1.5 MB Download da22wt690660w1d12u454c03trim-labels_PEP.am, Segmentation, 1.1 MB Download da22wt690660w1d12u454c03trim-labels_PEP.surf, Segmentation, 19.0 MB Download da22wt690660w1d12u454c03trim-labelsSlays.am, Segmentation, 1.6 MB Download da22wt690660w1d12u454c03trim-labelsSlays.surf, Segmentation, 42.6 MB Download Fig3a22wt.hx, Segmentation, 5.8 kB Download Fig3a22wtFL.surf, Segmentation, 261.2 kB Download Fig3a22wtIM.am, Segmentation, 1.1 MB Download Fig3a22wtIM.surf, Segmentation, 317.0 kB Download Fig3a22wtOM.am, Segmentation, 1.2 MB Download Fig3a22wtOM.surf, Segmentation, 3.2 MB DownloadAdditional details
- DOI
- 10.22002/81xvy-m7k27
- TILT_SERIES_ID
- zla2006-06-07-2
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
- Collected
-
2006-06-07
- Created
-
2008-07-01
- Updated
-
2009-07-10