Myxococcus xanthus pilM-YFP
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2014-05-14
Data Taken By: Yi-Wei Chang
Species / Specimen: Myxococcus xanthus
Strain:
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -8.0 μm, magnification: 26000x.
Microscope: Janelia Titan
Acquisition Software: Undefined
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain form Lotte Sogaard-Andersen
Purification / Growth Conditions / Treatment: Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.9
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated 10 nm gold. -4 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.
ycw2014-05-14-6_slicer5153.jpg: Extracellular Vesicles ycw2014-05-14-6_slicer5155.jpg: Unknown
Attached Files
20140514_Janelia_YWC096_10006_part131_2.rec, Reconstruction (Pixel Size 1.32 nm), 688.9 MB
20140514_Janelia_YWC096_10006.mrc, Tilt Series (Pixel Size 0.33 nm), 3.6 GB
Files
keyimg_ycw2014-05-14-6.jpg
Files
(4.3 GB)
| Name | Size | |
|---|---|---|
| 3.6 GB | (external) Download | |
| 688.9 MB | (external) Download | |
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md5:aec8eec18f682dff157623dc14c5b4a2
|
996.5 kB | Preview Download |
|
md5:e367746da613b4e99caea042f3d84588
|
25.8 MB | Preview Download |
|
md5:f0a3b278eb07c7164f19fb0cac8d7c7c
|
361.2 kB | Preview Download |
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md5:34c18955f566368c2bde41066ec75e1a
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359.3 kB | Preview Download |
Additional details
Identifiers
- DOI
- 10.22002/836wa-tm213
- TILT_SERIES_ID
- ycw2014-05-14-6
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2014-05-14
- Created
-
2014-05-15
- Updated
-
2014-07-08