Myxococcus xanthus PilP-sfGFP
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2014-03-08
Data Taken By: Yi-Wei Chang
Species / Specimen: Myxococcus xanthus
Strain:
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -8.0 μm, magnification: 26000x.
Microscope: Janelia Titan
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain form Lotte Sogaard-Andersen
Purification / Growth Conditions / Treatment: Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.8
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.
ycw2014-03-08-9_slicer3780.jpg: Myxococcus xanthus. Half fill, half empty vesicles. ycw2014-03-08-9_slicer7509.jpg: T6SS in Myxococcus xanthus ycw2014-03-08-9_slicer7510.jpg: unknown tube in Myxococcus xanthus delta-pilT
Attached Files
20140308_YWC093_10014.mrc, Tilt Series (Pixel Size 0.42 nm), 3.6 GB
20140308_YWC093_10014_full.rec, Reconstruction (Pixel Size 1.68 nm), 688.9 MB
Files
keyimg_ycw2014-03-08-9.jpg
Files
(4.3 GB)
| Name | Size | |
|---|---|---|
| 3.6 GB | (external) Download | |
| 688.9 MB | (external) Download | |
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md5:ad6ad1b65fa8dae5a70a94110c03404a
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1.0 MB | Preview Download |
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md5:4ba9646f1411c6d36897f5879400770b
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24.4 MB | Preview Download |
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md5:6bef0a4cbac02f38f7eddd56ed9c576c
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403.7 kB | Preview Download |
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md5:4191ee7c5902828e8cd72c038d208683
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316.9 kB | Preview Download |
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md5:dc0d796f0c77eed26b682c03b3e53f8f
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6.3 kB | Preview Download |
Additional details
Identifiers
- DOI
- 10.22002/85xe1-gp959
- TILT_SERIES_ID
- ycw2014-03-08-9
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2014-03-08
- Created
-
2014-03-11
- Updated
-
2014-07-18