Published 2020 | Version v1
Dataset Open

U937 JV9298

Creators

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

Legionella pneumophila JV9298 DotF-GFP DotB E191K

Technical info

Tilt Series Date: 2020-12-12

Data Taken By: Yuxi Liu

Species / Specimen: Legionella pneumophila

Strain: Philadelphia-1_Lp02

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -6.0 μm, magnification: 33000x.

Acquisition Software: Serial EM

Upload Method: rundir

Processing Software Used: imod, tomo3d

Collaborators and Roles: Sample preparation, data collection & processing: Yuxi Liu

Purification / Growth Conditions / Treatment: 1h infection, grids coated with 25ug/mL vitronectin

Sample Preparation: Quantifoil R2/2 gold finder grid, 100% humidity, 22deg C, manual blotting

Attached Files

U937_9298_tomo_16_bin2_SIRT_1k.rec, Reconstruction (Pixel Size 1.06 nm), 4.1 GB

U937_9298_tomo_16_bin2.st, Tilt Series (Pixel Size 0.53 nm), 483.2 MB

Files

keyimg_ylx2020-12-12-20.jpg

Files (4.6 GB)

Name Size
md5:7056b7e008319c75e1afcb2427a64672
641.7 kB Preview Download
md5:8fd86e29bbc425428ae78a364f328ba3
10.7 MB Preview Download
483.2 MB (external) Download
4.1 GB (external) Download

Additional details

Identifiers

DOI
10.22002/86j2c-a3g84
TILT_SERIES_ID
ylx2020-12-12-20

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2020-12-12
Created
2021-07-26
Updated
2021-07-26