Hippocampal Neurons (cultured)
Contributors
Researchers:
Description
Technical info
Tilt Series Date: 2015-02-16
Data Taken By: Matt Swulius
Species / Specimen: Rat Hippocampal Neuron (cultured)
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Xiawei Zhuang, Ruobo Zhou (cultured neurons and STORM imaging)
Purification / Growth Conditions / Treatment: Plunge frozen at DIV 12 Grids were treated with PDL prior to plating and 3 ul concentrated gold suspended in growth media was added to grid before freezing.
Sample Preparation: Grids were plunge froze in a vitrobot at 95 % humidity adn 22 C. They were manually blotted from behind.
Attached Files
021615_hipp_neuron_hole90010.mrc, Tilt Series (Pixel Size 0.41 nm), 3.4 GB
021615_hipp_neuron_hole90010_full.rec, Reconstruction (Pixel Size 1.64 nm), 445.4 MB
Files
keyimg_mst2015-02-16-16.jpg
Additional details
Identifiers
- DOI
- 10.22002/8be6b-pde33
- TILT_SERIES_ID
- mst2015-02-16-16
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2015-02-16
- Created
-
2015-02-17
- Updated
-
2015-02-20