Treponema primitia
Contributors
Researchers:
Description
Technical info
Tilt Series Date: 2005-09-11
Data Taken By: Gavin Murphy
Species / Specimen: Treponema primitia
Strain: ZAS-2
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1°, constant angular increment, dosage: 30.0 eV/Ų, defocus: -12.0 μm, magnification: 0x.
Acquisition Software: UCSF tomo
Upload Method: rundir
Processing Software Used: imod, bsoft
Collaborators and Roles: Cells provided by Eric Matson, Jared Leadbetter; Data collected and processed by Gavin Murphy
Purification / Growth Conditions / Treatment: Cells grown in standard media around exponential growth. Removed from anaerobic tube with syringe. Uncentrifuged.
Sample Preparation: quantifoil grid, 10nm gold, 100% humidity...
T4_050811_tilt.jpg: * associated to rawdata T4_050911_rec.jpg: file associated to 3D Image #53
Attached Files
T4_S552G2_22kx14d130e.mrc.gz, Tilt Series (Pixel Size 0.98 nm), 798.7 MB
T4B2s.mrc, Reconstruction (Pixel Size 0.0 nm), 312.1 MB
T4B2sBB_28_part.pif, Reconstruction (Pixel Size 0.0 nm), 352.3 kB
gme2005-09-11-4_slicer19173.png, REZ with faint flow lines pointing (mostly) parallel to the axis of the cell. spacing between filaments in this image appears to be 8-9nm with large variations (assuming voxel width=1.96nm). -andrew, 619.2 kB
Files
gme2005-09-11-4_slicer19173.png
- This file is an external reference and not stored directly in this repository. To access its content, please download it and open it locally.
Files
(1.1 GB)
| Name | Size | |
|---|---|---|
| 619.2 kB | (external) Download | |
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md5:e466b0ea493ba84791597a655298ed03
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738.5 kB | Preview Download |
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md5:afcc8df43e365a8a2a44e60984bccf08
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5.3 MB | Preview Download |
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md5:193a31f1acec0e46cb25dadf261b806c
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5.0 kB | Preview Download |
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md5:6d8d1e2bd75eb239cd6912450953d107
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6.9 kB | Preview Download |
| 798.7 MB | (external) Download | |
| 312.1 MB | (external) Download | |
| 352.3 kB | (external) Download |
Additional details
Identifiers
- DOI
- 10.22002/8d2zq-spn45
- TILT_SERIES_ID
- gme2005-09-11-4
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2005-09-11
- Created
-
2008-06-18
- Updated
-
2017-12-07