Published 2015 | Version v1
Dataset Open

Crosslinked HIV-1 cores TRIM5α

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

Hyperstable purified HIV-1 cores crosslinked with TRIM5α

Technical info

Tilt Series Date: 2015-01-03

Data Taken By: Stephen Carter

Species / Specimen: HIV cores

Strain: None

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 109.0 eV/Ų, defocus: -5.0 μm, magnification: 41000x.

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: IMOD and Raptor

Collaborators and Roles: Wes Sundquist

Purification / Growth Conditions / Treatment: Grids frozen in Utah

Sample Preparation: Non infectious HIV-1 cores decorated with TRIM5α. 0.3 µM sulfo-EGS

Attached Files

tomography0022.mrc, Tilt Series (Pixel Size 0.26 nm), 3.4 GB

tomography0022_part121_7.rec, Reconstruction (Pixel Size 1.04 nm), 445.4 MB

Files

keyimg_scd2015-01-03-21.jpg

Files (3.9 GB)

Name Size
md5:c504f51dd05a36980766572cdafd9b9e
1.1 MB Preview Download
md5:501110f23b88857b00375863cf1a921e
14.0 MB Preview Download
3.4 GB (external) Download
445.4 MB (external) Download

Additional details

Identifiers

DOI
10.22002/8dnee-zkk77
TILT_SERIES_ID
scd2015-01-03-21

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2015-01-03
Created
2015-01-03
Updated
2016-03-01