Sulfolobus solfac.P2+STIV
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2014-01-14
Data Taken By: Rasika Ramdasi
Species / Specimen: Sulfolobus solfataricus
Strain: P2
Tilt Series Settings: Single Axis, tilt range: (-55.0°, 55.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: With Jamie Snyder at MSU, Montana.
Sample Preparation: S. solfataricus strain P2 cultures were infected with STIV as previously described (Ortmann et al., 2006). Sulfolobus were grown up from glycerol stock in media 182 (pH 3.5), 80°C and passage once. The cultures were passed second time to media 182 (pH 2.5) and infected with STIV at MOI ~10 at OD 650 nm ~0.2. At 10 hour Post infection, cells were concentrated ~20 fold by centrifugation.
Attached Files
stiv10h40019.mrc, Tilt Series (Pixel Size 0.48 nm), 3.2 GB
stiv10h40019_full.rec, Reconstruction (Pixel Size 1.92 nm), 623.6 MB
Files
keyimg_rra2014-01-14-29.jpg
Additional details
Identifiers
- DOI
- 10.22002/8gnmb-t3e28
- TILT_SERIES_ID
- rra2014-01-14-29
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2014-01-14
- Created
-
2014-01-15
- Updated
-
2014-01-15