Bdellovibrio attack-phase
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2012-11-14
Data Taken By: Yi-Wei Chang
Species / Specimen: Bdellovibrio bacteriovorus
Strain: HD100
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -10.0 μm, magnification: 34000x.
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: UCSFTomo
Collaborators and Roles: Strain from Liz Sockett
Purification / Growth Conditions / Treatment: Cells grown in S17-1 prey solution at 30 degrees. OD600=0.2
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix.
Attached Files
Bdellovibrio_wt_20121114_0030006.mrc, Tilt Series (Pixel Size 0.33 nm), 3.4 GB
Bdellovibrio_wt_20121114_0030006_part122_4.rec, Reconstruction (Pixel Size 1.32 nm), 441.7 MB
Files
keyimg_ycw2012-11-14-47.jpg
Additional details
Identifiers
- DOI
- 10.22002/8j7xw-09882
- TILT_SERIES_ID
- ycw2012-11-14-47
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2012-11-14
- Created
-
2012-11-16
- Updated
-
2012-11-29