Hylemonella gracilis

Description

Technical info

Tilt Series Date: 2012-09-23

Data Taken By: Yi-Wei Chang

Species / Specimen: Hylemonella gracilis

Strain: ATCC 19624

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 112.0 eV/Ų, defocus: -15.0 μm, magnification: 22500x.

Acquisition Software: UCSF Tomo

Upload Method: pipeline

Processing Software Used: RAPTOR

Purification / Growth Conditions / Treatment: Cell grown 48 hr in Broth 233 at 26°C without antibiotics, Final OD600 < 0.1, Spun down by 1000 rcf for 5 min and concentrated by ~10x for plunge freezing

Sample Preparation: Vitrobot setting: 100% humidity, 4 ul sample, 10 nm BSA treated gold, -3 offset, 4 s blot time, no drain, ethane/propane mix

ycw2012-09-23-38_slicer4988.jpg: Flagellar motor ycw2012-09-23-38_slicer4990.jpg: REZ ycw2012-09-23-38_slicer4992.jpg: Budding Membrane . Nucleoid

Attached Files

20120923_Hylemonella_30002.mrc, Tilt Series (Pixel Size 0.49 nm), 3.4 GB

20120923_Hylemonella_30002_part122_3.rec, Reconstruction (Pixel Size 1.96 nm), 441.7 MB