Sulfolobus cells synchronized for division
Contributors
Researchers:
Description
Technical info
Tilt Series Date: 2010-12-03
Data Taken By: Megan Dobro
Species / Specimen: Sulfolobus acidocaldarius
Strain:
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1°, constant angular increment, dosage: 2.7 eV/Ų, defocus: -15.0 μm, magnification: 18000x.
Acquisition Software: Leginon
Upload Method: rundir
Processing Software Used: Raptor
Collaborators and Roles: grown and synchronized by Rachel Samson in Stephen Bell's lab, Oxford University
Purification / Growth Conditions / Treatment: grown in Brock's Medium at 80C, synchronize with Baby Machine
Sample Preparation: frozen at Kay Grunewald's lab. Harrick plasma cleaner. Blotted on both sides, 4 sec, sponge for humidity
mdj2010-12-03-10_slicer3752.jpg: archellum mdj2010-12-03-10_slicer3753.jpg: archellum- basal body?
Attached Files
10dec03f_Box13grid1_005new.mrc, Tilt Series (Pixel Size 1.258 nm), 1.1 GB
10dec03f_Box13grid1_005new_part.rec, Reconstruction (Pixel Size 2.38 nm), 1.0 GB
Files
keyimg_mdj2010-12-03-10.jpg
Files
(2.2 GB)
| Name | Size | |
|---|---|---|
| 1.1 GB | (external) Download | |
| 1.0 GB | (external) Download | |
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md5:457176e18e74dc7e7ed40e9e5b55aee3
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1.2 MB | Preview Download |
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md5:2d724758082d0512ef83f3cc8b45ecc0
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30.4 MB | Preview Download |
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md5:7f951e91f05936492bad6aeeae11fc5a
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210.9 kB | Preview Download |
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md5:b7453ef1e70a6e818d73cadf2c519508
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143.4 kB | Preview Download |
Additional details
Identifiers
- DOI
- 10.22002/8rqfz-nhm60
- TILT_SERIES_ID
- mdj2010-12-03-10
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2010-12-03
- Created
-
2012-07-09
- Updated
-
2014-03-12