Caltech Tomography Archive
Published 2015 | Version v1
Dataset Open

pED208_pilated

Tocheva, Elitza1
  • 1. ROR icon California Institute of Technology

Description

E coli strain with plasmid pED208 overexpresses T4SS and F-like pili

Files

keyimg_etl2015-01-14-51.jpg
Files (13.8 MB)
Name Size
keymov_etl2015-01-14-51.mp4
md5:b07802268a6ca07cc06f6d310d27cc4a
12.8 MB Download
keyimg_etl2015-01-14-51.jpg
md5:77ce0ab4c69ab19842987b189671b801
1.0 MB Preview Download

Technical info

Tilt Series Date: 2015-01-14

Data Taken By: Elitza Tocheva

Species / Specimen: Escherichia coli

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Cells were obtained from Laura Frost

Purification / Growth Conditions / Treatment: o/n liquid LB

Sample Preparation: Vitrobot

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/etl2015-01-14-51/rawdata/

pED208a0051.mrc, Tilt Series (Pixel Size 0.5 nm), 3.4 GB Download

pED208a0051_full.rec, Reconstruction (Pixel Size 2.0 nm), 445.4 MB Download

Additional details

Identifiers Funding Dates
Created:
May 25, 2023
Modified:
August 26, 2025