Published 2017 | Version v1
Dataset Open

Staphylococcus aureus_WT

  • 1. ROR icon California Institute of Technology

Description

T7SS

Technical info

Tilt Series Date: 2017-06-28

Data Taken By: Debnath Ghosal

Species / Specimen: Unspecified

Strain: None

Tilt Series Settings: Single Axis, tilt range: (-50.0°, 50.0°), step: 1.5°, constant angular increment, dosage: 280.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain sent to us by Tracy Palmer, University of Dundee. Data collected and processed by Ki Woo and Debnath Ghosal.

Sample Preparation: Media: TSA 500g (Oxoid TSA and TSB from Thermo Fisher. 10525943), TBS 500g (10137562)Dilute an overnight culture 1/100Incubate it for 4 hr (--> OD 2)Vitrobot

Attached Files

Sau_WT0028.mrc, Tilt Series (Pixel Size 0.39 nm), 1.9 GB

Sau_WT0028_full.rec, Reconstruction (Pixel Size 1.56 nm), 445.4 MB

Files

keyimg_dga2017-06-28-29.jpg

Files (2.4 GB)

Name Size
md5:e9529f226f21712d3d841d006486add3
1.0 MB Preview Download
md5:e3a7a257d92df7756229df9bc5ccb2a0
10.7 MB Preview Download
1.9 GB (external) Download
445.4 MB (external) Download

Additional details

Identifiers

DOI
10.22002/8wnes-ebt68
TILT_SERIES_ID
dga2017-06-28-29

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2017-06-28
Created
2017-06-30
Updated
2017-07-05