Myxococcus xanthus delta-pilM
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2014-06-26
Data Taken By: Yi-Wei Chang
Species / Specimen: Myxococcus xanthus
Strain:
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -8.0 μm, magnification: 26000x.
Microscope: Janelia Titan
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain form Lotte Sogaard-Andersen
Purification / Growth Conditions / Treatment: Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.8
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.
ycw2014-06-26-12_slicer7604.jpg: filament Myxococcus xanthus ycw2014-06-26-12_slicer7612.jpg: filament? Myxococcus xanthus
Attached Files
20140626_YWC058_Janelia_20006.mrc, Tilt Series (Pixel Size 0.33 nm), 3.6 GB
20140626_YWC058_Janelia_20006_full.rec, Reconstruction (Pixel Size 1.32 nm), 688.9 MB
Files
keyimg_ycw2014-06-26-12.jpg
Files
(4.3 GB)
| Name | Size | |
|---|---|---|
| 3.6 GB | (external) Download | |
| 688.9 MB | (external) Download | |
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md5:5c78e40a67924d9c75abbbf33850b481
|
999.8 kB | Preview Download |
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md5:043b4f8c69b0d2c1fb6e6591bf3e5c0e
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25.3 MB | Preview Download |
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md5:30be2dde5e1794e00df2e877d4c7d3f6
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173.1 kB | Preview Download |
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md5:acbc7dce100916670a673cceb44fc3a4
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173.0 kB | Preview Download |
Additional details
Identifiers
- DOI
- 10.22002/8yn8n-v9545
- TILT_SERIES_ID
- ycw2014-06-26-12
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2014-06-26
- Created
-
2014-06-27
- Updated
-
2014-07-21