Published 2014
| Version v1
Dataset
Open
E. coli N050
Contributors
Researcher:
Description
Sandwich fusion to sfGFP. Wild Type MreB (no point mutations)
Technical info
Tilt Series Date: 2014-03-27
Data Taken By: Matt Swulius
Species / Specimen: Escherichia coli
Strain: NO50
Tilt Series Settings: Single Axis, tilt range: (-64.0°, 64.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Josh Schaevitz
Purification / Growth Conditions / Treatment: Grown at 37 to OD600 = 0.3 then plunge frozen.
Sample Preparation: Plunge Frozen
Attached Files
032714_NO500015.mrc, Tilt Series (Pixel Size 0.48 nm), 3.7 GB
032714_NO500015_full.rec, Reconstruction (Pixel Size 0.96 nm), 3.6 GB
Files
keyimg_mst2014-03-27-8.jpg
Additional details
Identifiers
- DOI
- 10.22002/90zar-w0362
- TILT_SERIES_ID
- mst2014-03-27-8
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2014-03-27
- Created
-
2014-03-27
- Updated
-
2014-03-31