Planktonic cell of Proteus
Description
Technical info
Tilt Series Date: 2015-12-26
Data Taken By: Qing Yao
Species / Specimen: Proteus mirabilis
Strain: HI4320
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 195.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Douglas B. Weibel lab, Wisconsin-Madison university
Purification / Growth Conditions / Treatment: OD=6, 4 ul loading. bloting condition 6s force/ 3s time/ 1s dry time, QUANTIFOIL® – Holey Carbon Films 200 mesh
Sample Preparation: OD=6, 4 ul loading. bloting condition 6s force/ 3s time/ 1s dry time, QUANTIFOIL® – Holey Carbon Films 200 mesh
Attached Files
proteus30017_part121_4.rec, Reconstruction (Pixel Size 1.92 nm), 445.4 MB
proteus30017.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB
Files
keyimg_qya2015-12-26-17.jpg
Additional details
Identifiers
- DOI
- 10.22002/90zzg-h8r09
- TILT_SERIES_ID
- qya2015-12-26-17
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2015-12-26
- Created
-
2015-12-27
- Updated
-
2022-10-12