V. cholerae CF59 - ∆tcpB pMT5

Description

Technical info

Tilt Series Date: 2015-06-17

Data Taken By: Yi-Wei Chang

Species / Specimen: Vibrio cholerae

Strain: O395-N1

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: reupload_downloaded

Processing Software Used: SIRT

Collaborators and Roles: Strain from Ronald K. Taylor

Purification / Growth Conditions / Treatment: ON cultures of Vibrio cholerae O395 NI pMT5 grown in LB pH 6.5, 1 mM IPTG and 100 ug/mL ampicilin

Sample Preparation: Vitrobot (MK iv) settings: 100% humidity, 4µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.

Attached Files

20150617_AK08_10031_SIRT_2K.rec, Reconstruction (Pixel Size 0.0 nm), 5.7 GB

20150617_AK08_10031.mrc, Tilt Series (Pixel Size 0.39 nm), 3.4 GB

20150617_AK08_10031_SIRT_2K_cropped.rec, magenta arrow: strange hollow spheres . . green arrow: filamentous structure (from some viewing angles resembles a twisted ladder or a washboard). This structure may contain DNA. . . Both structures are also visible in: ab2015-06-02-1 ab2015-06-02-29 . [2015-11-21 00:18:08] -andrew, 2.1 GB

ycw2015-06-17-31_slicer14024.png, 556.5 kB