O. tauri arrested w/ MG132

Description

Technical info

Tilt Series Date: 2008-03-21

Data Taken By: Lu Gan

Species / Specimen: Ostreococcus tauri

Strain: RCC745

Tilt Series Settings: Single Axis, tilt range: (-55.0°, 55.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -14.0 μm, magnification: 0x.

Acquisition Software: UCSF Tomo

Upload Method: rundir

Processing Software Used: imod

Collaborators and Roles: prepared by Lu Gan at Caltech; data collected by Lu Gan; processed by Lu Gan

Purification / Growth Conditions / Treatment: Block at G1/S 12h w/Aphidicolin, block 3h w/Propyzamide, block 6h

Sample Preparation: C-flat LF 4/2 thick carbon, BSA treated 10nm gold, 100% humidity, -2mm offset,

Attached Files

RCC745_MG132_04.mrc, Tilt Series (Pixel Size 1.59 nm), 939.7 MB

RCC745_MG132_04.rec, Reconstruction (Pixel Size 3.18 nm), 274.5 MB